| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morelli, Brennan Q (14)
|
MRHAUP |
24.82 |
24.29 |
-0.53 |
| 1 |
Vapnyar, Nicholas J (14)
|
MRTS |
24.44 |
24.64 |
0.20 |
| 2 |
Acevedo, Lucas M (14)
|
MRFA |
24.52 |
24.71 |
0.19 |
| 2 |
Catalanotto, Michael F (14)
|
MRSSC |
26.73 |
26.80 |
0.07 |
| 3 |
Chavez, Nicholas (14)
|
MREHY |
24.65 |
24.76 |
0.11 |
| 3 |
Przysowa, Dylan K (14)
|
MRFA |
25.63 |
27.00 |
1.37 |
| 4 |
Baker, Noah (14)
|
MRHYB |
25.23 |
24.97 |
-0.26 |
| 4 |
Fidos, Nicolas J (14)
|
MRTVSC |
26.47 |
27.06 |
0.59 |
| 5 |
Dwyer, William (14)
|
MRHYB |
25.42 |
24.98 |
-0.44 |
| 5 |
Naseer, Rehan * (14)
|
MRTS |
26.72 |
27.54 |
0.82 |
| 6 |
Chang, Caiden (14)
|
MRLIAC |
25.47 |
25.02 |
-0.45 |
| 7 |
Li, Isaac (14)
|
MRLIAC |
24.89 |
25.03 |
0.14 |
| 8 |
Duong, Kevin E (14)
|
MRTVSC |
25.27 |
25.15 |
-0.12 |
| 9 |
Arrascue, Daniel (14)
|
MRTS |
26.72 |
25.18 |
-1.54 |
| 10 |
Wong, Alexander (14)
|
MRTS |
25.01 |
25.28 |
0.27 |
| 11 |
Marrero, Milo M (14)
|
MRHYB |
25.44 |
25.40 |
-0.04 |
| 12 |
Korica, Marko (14)
|
MRLIAC |
25.94 |
25.54 |
-0.40 |
| 13 |
Ip, LokShing (14)
|
MRLIAC |
25.12 |
25.66 |
0.54 |
| 14 |
Baranowski, Bryce B (14)
|
MRTVSC |
25.06 |
25.68 |
0.62 |
| 15 |
Makkar, Aayush D (14)
|
MRTS |
24.95 |
25.75 |
0.80 |
| 16 |
Ahn, Benjamin S (14)
|
MRHYB |
25.81 |
25.79 |
-0.02 |
| 17 |
Gedeon Jr, Israel (14)
|
MRTVSC |
25.71 |
25.80 |
0.09 |
| 18 |
Raber, James (14)
|
MRENAC |
25.69 |
25.82 |
0.13 |
| 19 |
Stanislawski, Christopher J (14)
|
MRLIAC |
26.33 |
25.85 |
-0.48 |
| 20 |
Owens, Jace (14)
|
MRFA |
26.14 |
26.10 |
-0.04 |
| 21 |
Kuang, Gavin (14)
|
MRENAC |
26.36 |
26.26 |
-0.10 |
| 22 |
Shemetov, Ivan T (14)
|
MRFA |
26.15 |
26.51 |
0.36 |
|
Kimmy, Nathanael E (14)
|
MRTVSC |
25.97 |
DQ |
- |